As a new type foreign trade service provider driven by both technology transformation and foreign trade services, HiSiaddi has built a "1+2+3+4=1" service system and can supply acetyl octapeptide sourced from multiple well-known original manufacturers. As a technology-driven foreign trade enterprise, HiSiaddi has repeatedly collaborated with factories on technology conversion and accurately captured market demands to resolve customized production demands for acetyl octapeptide from clients. Below is a case of HiSiaddi addressing customized production requirements for acetyl octapeptide.
If you need more customized services, please contact HiSiaddi customer service.
The purchaser is DermAlma S.L., a Spanish enterprise located in the high-tech beauty and biological industrial park of Barcelona. For over 30 years, it has focused on the R&D and production of high-end anti-aging skincare products for European medical aesthetic salons. Its products are exclusively supplied to premium medical aesthetic clinics and SPA salons across Spain, Portugal and Italy, complying with EU CE cosmetic raw material access standards. Its flagship firming anti-wrinkle serum relies on Acetyl Octapeptide-3 (CAS: 868844-74-0), an upgraded high-end anti-wrinkle active peptide commonly known as topical high-potency botulinum peptide for diminishing dynamic expression lines.
The client previously sourced high-purity octapeptide from Spain’s Lipotec all year round with an annual fixed purchase volume of 4.2kg. However, surging European raw material prices, a 120-day extended customized production lead time from the original supplier, and the need for non-standard customized octapeptide with tailored indicators for its new product line prompted the client to launch a localization project for raw material customization in China.
The client independently contacted three domestic peptide synthesis factories in China at the early stage, yet general mass-produced products only reached an HPLC purity of 97%~97.5%, failing to meet the client’s customized thresholds: HPLC ≥99.2%, single missing peptide impurity ≤0.1%, trifluoroacetic acid (TFA) residue ＜200ppm, peptide content ≥85%, endotoxin-free, and acetate salt form instead of TFA salt. All domestic factories only mass-produce conventional TFA salt octapeptide and lacked capabilities to switch to acetate salt formation processes. The client spent EUR 23,000 on sample testing fees, yet multiple batches failed all inspections, delaying the launch of its new product line.
Recommended by the Spanish Cosmetic Raw Materials Association, the client fully entrusted Shanghai HiSiaddi Technology to deliver full-cycle customized production. Supported by HiSiaddi’s peptide R&D engineers and cosmetic compliance specialists, we resolved implementation challenges from four dimensions: synthetic route optimization, refined process modification, salt formation system adjustment and customized quality control. After lab-scale samples passed all tests, a long-term customized supply framework with an annual volume of 4.2kg was finalized.
Product delivered: High-purity acetate salt acetyl octapeptide white crystalline powder, vacuum foil light-proof packaging, paired with bilingual COA, original HPLC spectra, endotoxin test reports and EU CPNP compliance documents.
Domestic industrial mass production of acetyl octapeptide generally uses trifluoroacetic acid (TFA) for deprotection and salt formation, leaving residual TFA solvent in finished products. EU high-end medical aesthetic skincare regulations limit TFA residue in raw materials to ≤200ppm, while conventional processes result in TFA residues of 800~1500ppm. The client’s formula strictly requires acetate salt formation with zero TFA residue. Conventional mass production processes cannot directly switch salt formation systems, and process modification requires reconstructing the entire deprotection, neutralization and crystallization procedures.
Acetyl octapeptide is synthesized via solid-phase stepwise coupling of 8 amino acids across more than 30 synthetic steps, easily generating missing peptide impurities such as alanine-deficient and arginine-deficient fragments. Conventional domestic products feature single impurity peaks ≤1.0%, while the client’s customized standard caps single unknown impurities at ≤0.1% and total impurities at ≤0.8%. Single-pass reverse-phase chromatography cannot separate trace isomeric impurities.
The raw material is used for invasive medical aesthetic infusion serums, requiring endotoxin ＜0.1EU/mg and total bacterial count ＜10CFU/g. Most domestic peptide factories only control physical and chemical purity, lacking sterile refining and sterile packaging workshops. Their factory inspection reports exclude full microbial and pyrogen testing data, making their products ineligible for EU medical aesthetic product filing.
The 4.2kg annual order is split into 6 small production batches. General production lines are dedicated to mass manufacturing TFA salt products; switching to acetate salt formation and multi-stage gradient purification requires emptying reaction kettles and replacing all chromatographic packing materials. Most manufacturers either mark up prices by over 70% or refuse customized non-standard salt type orders due to high modification costs.
HiSiaddi formed a dedicated peptide technical team and collaborated with a GMP-certified domestic peptide pilot production base for cosmetic raw materials, completing the full workflow of lab-scale optimization, pilot mass production, full-item testing and customs clearance shipment within 52 days.
We abandoned the traditional strong acid TFA deprotection route and adopted stepwise mild alkaline deprotection to remove side-chain protecting groups. After deprotection, low-temperature acetate buffer solution was used for neutralization and crystallization to replace TFA salt formation with acetate salt formation. Temperature control was implemented throughout segmented processes to eliminate solvent residues. Finished products tested negative for TFA, with acetate content stabilized at 7.2% (within the client’s required range of 5%~12%), perfectly matching the pH requirements of the formula.
We optimized raw material feeding ratios for solid-phase peptide synthesis and applied real-time Kaiser ninhydrin monitoring to track reaction conversion rates after each amino acid coupling step. Under-converted raw materials were re-fed to reduce missing peptide generation at the source. Crude products underwent secondary purification via C18 reverse-phase gradient chromatography and low-temperature recrystallization, with main peak fractions collected separately. The final finished product achieved HPLC purity of 99.35%, maximum single impurity of 0.072% and total impurities of 0.63%, exceeding the client’s upper customized limits.
We coordinated the partner factory’s Class 10,000 clean workshop for post-process refining, vacuum freeze-drying and sterile packaging, with temperature-controlled and light-shielded freeze-drying environments and an additional sterile filtration step for finished products. We partnered with a CNAS-accredited third-party laboratory for full-item testing of endotoxins, microorganisms and heavy metals, issuing test reports formatted for EU cosmetic registration. The measured endotoxin level of finished products reached 0.06EU/mg, meeting the standards for invasive medical aesthetic raw materials.
HiSiaddi adopted a production mode of 5g lab samples → 200g pilot batches → split mass production. First, 5g samples were sent to the client’s Spanish R&D laboratory for full-item re-testing. After NMR, liquid chromatography and microbial tests all passed, split batch production commenced. Customized vacuum light-proof aluminum foil individual small packaging was applied to prevent moisture-induced degradation during long-distance ocean transportation.
1. The first 700g customized acetate salt acetyl octapeptide was shipped via international cold chain air freight to Barcelona. All indicators passed full re-testing at Spanish laboratories, enabling its formal addition to new medical aesthetic firming serum mass production. The new product launched on schedule across Spanish medical aesthetic salons. Compared with the original European imported raw materials, overall procurement costs dropped by 37%, and delivery lead time was shortened from 120 days to 45 days.
2. One month after acceptance of the first shipment, DermAlma officially signed a long-term customized supply framework for an annual volume of 4.2kg, agreeing to entrust HiSiaddi with customized development of compound peptides (copper peptide, pentapeptide) for subsequent new products, with internal raw material indicators adjusted annually in line with updated EU regulations.
3. Leveraging this benchmark case and the client’s European salon resources, HiSiaddi expanded its customer base to two mid-to-high-end clients: a premium medical aesthetic laboratory in Paris, France and a local skincare research institute in Portugal, replicating the full-chain peptide customization service model.
As a core high-activity anti-aging peptide for premium medical aesthetic products, acetyl octapeptide features massive technical gaps between conventional daily chemical grade and customized medical aesthetic grade products in synthesis processes, salt formation types, impurity control and biosafety. Most domestic peptide manufacturers focus on low-cost mass production of TFA salt general-grade products, lacking capabilities for refined customization of non-standard salt types and pharmaceutical-grade materials. Raw material indicators for European mid-to-high-end medical aesthetic brands are bound by EU cosmetic regulations and salon product formulas. Independent raw material sourcing often leads to unqualified processes, substantial sample testing cost losses and delayed new product launches.
Moving beyond traditional raw material matching foreign trade, HiSiaddi centers on peptide synthesis technology, customized process optimization and full-chain compliance support to connect process modification, purification optimization, clean packaging and cross-border export workflows. We help overseas premium brands source customized domestic raw materials and support domestic high-end peptide raw materials to enter European medical aesthetic supply chains.
If you need more customized services, please contact HiSiaddi customer service.
+86-13120989737 
info@hisiaddi.com 
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