HiSiaddi is an innovative foreign trade service provider driven by both technology commercialization and export business. We have established a "1+2+3+4=1" service system and can supply lactase from multiple well-known original manufacturers. As an export trader with in-house R&D capabilities, HiSiaddi has repeatedly resolved customized lactase demands for clients through technological cooperation with manufacturers and precise market insight. Below is a case of customized lactase development delivered by HiSiaddi.
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Buyer: AlpenDairy, a premium organic dairy group based in the Swiss Alps, a benchmark mid-to-high-end dairy enterprise in Western Europe. Its core products include organic pasteurized fresh milk, high-protein Greek yogurt, and lactose-free infant formula dairy products, sold to high-end organic supermarkets, maternal and infant retail chains, and star hotel ingredient suppliers across Switzerland, Austria and Liechtenstein. All its products comply with EU EFSA, FCC, Swiss FOODS and KOSHER clean label standards, with an annual lactase procurement volume of 22 tons.
The group previously purchased customized lactase from Novozymes Denmark for a long time. Driven by surging European raw material prices and prolonged delivery cycles, it planned to source customized lactase exclusively from China. After contacting multiple domestic enzyme manufacturers, it found standard mass-produced models incompatible with its special production processes, and entrusted HiSiaddi to deliver the full customized development process via foreign trade services.
1. Customized enzyme activity specification: Neutral lactase with a targeted activity of 85,000 NLU/g. Mainstream mass-produced commercial grades only offer fixed levels of 50,000 NLU/g or 100,000 NLU/g without this precise intermediate grade; enzyme activity fluctuation is strictly controlled within ±3%.
2. Customized enzymatic parameters: Optimal pH range of 6.2–6.6 and applicable temperature of 32–42°C, matching the client’s in-situ lactose hydrolysis process for fresh milk at ambient temperature. Enzyme activity retention shall be no less than 90% after short-time pasteurization pretreatment at 45°C to avoid thermal deactivation.
3. Customized carrier formulation: Non-GMO corn maltodextrin carrier, zero dairy-derived ingredients, no exogenous protease/amylase impurities, to meet organic clean label standards and prevent off-flavors that interfere with yogurt fermentation.
4. Customized dosage form: Cold-water instant microcapsule powder with low dust generation, compatible with automated pipeline online feeding without clogging precision filter pipelines.
5. Customized compliance documentation: Complete Swiss import-specific test reports, JECFA toxicology dossiers and FCC specification sheets attached to finished goods.
Three leading domestic lactase manufacturers only offered standardized mass-produced grades and were merely willing to make minor carrier adjustments, unable to conduct targeted strain modification or optimize post-extraction purification processes:
1. Enzyme activity was either fixed at 100,000 NLU/g (too high) or 50,000 NLU/g (too low), failing to lock the precise target of 85,000 NLU/g. Excessive enzyme activity fluctuation caused inconsistent lactose hydrolysis rates in fresh milk, making it impossible to meet the EU standard of residual lactose <0.01% for "lactose-free" labeling.
2. Conventional bulk products contained trace residual miscellaneous proteases, which damaged the protein structure of yogurt and resulted in product stratification and rancid off-flavors.
3. Ordinary fine powder generated heavy dust, clogging the client’s automated closed feeding pipeline and bringing high production shutdown risks. Manufacturers lacked dedicated R&D teams for custom orders. The client invested nearly 10,000 Swiss Francs on self-developed trial samples, all of which failed, delaying the scheduled launch of new products.
HiSiaddi horizontally screened domestic biotech factories specializing in customized enzyme development, split client indicators into segmented R&D parameters, and coordinated manufacturers to adjust four core processes: strain breeding → liquid fermentation → membrane separation purification → spray microcapsule encapsulation.
· Fermentation: Optimize Kluyveromyces fermentation medium and temperature control curves to precisely regulate enzyme yield and lock the target baseline enzyme activity.
· Purification: Adopt two-stage nanofiltration membrane purification to remove miscellaneous enzyme components and strictly control residual heteroproteins and exogenous enzymes, eliminating off-flavors in finished products.
· Post-processing: Deploy customized low-temperature spray granulation and microcapsule coating technology to achieve instant full dissolution in cold water with zero dust.
1. Laboratory samples (3 batches × 500g): Test enzyme activity, pH tolerance, thermal stability and impurity indicators for each batch. HiSiaddi coordinated parallel testing at the client’s Swiss laboratory. After 3 rounds of fermentation parameter fine-tuning, enzyme activity stabilized between 84,100–86,300 NLU/g with fluctuation below 2.8%, fully meeting requirements.
2. Pilot samples (50kg): Full-process small-batch production on the client’s Swiss production line. Lactose residue in fresh milk stabilized at approximately 0.007%, yogurt showed no stratification or abnormal flavors, and feeding proceeded smoothly without pipeline clogging.
3. Mass production trial order (1.2 tons): Full-scale production on commercial lines. CNAS-accredited laboratories conducted comprehensive testing per Swiss import standards, with all heavy metal, microbial and allergen indicators fully compliant.
1. HiSiaddi assisted manufacturers in developing trilingual (Chinese, English, German) product specification sheets and batch traceability documents per Swiss food safety requirements, and processed FCC conformity reports and special third-party testing dossiers.
2. Outer packaging was customized in compliance with Swiss labeling laws, clearly marking enzyme activity value, strain source, carrier composition and compliance codes to pass customs inspection in Switzerland.
1. Order Execution: After successful trial production, the client signed a 22-ton annual framework procurement contract with monthly delivery in 12 batches. The customized lactase reduced procurement costs by 31% compared with imported European raw materials.
2. End Product Launch: Supported by the customized lactase, three new organic lactose-free fresh milk and infant yogurt lines were fully launched across high-end supermarkets nationwide in Switzerland. New product revenue increased by 24% within half a year of launch.
3. Benefits for Manufacturers: Based on this benchmark high-end Swiss order, the factory finalized a mature mass production process for the exclusive 85,000 NLU/g lactase grade, forming a proprietary high-end customized product line. Subsequent inquiries for customized lactase were received from two organic dairy enterprises in Austria and Luxembourg.
4. Business Growth for HiSiaddi: Established a standardized customized enzyme service model covering "parameter decomposition – process customization – multi-round sampling – compliance document support", consolidating its presence in the high-end customized food enzyme export track targeting European organic markets.
Most domestic enzyme manufacturers focus on standardized bulk products and lack refined customized R&D capabilities for high-end overseas clients, limited to simple carrier adjustments. High-end organic dairy manufacturers in Europe adopt highly refined production processes with personalized specifications for lactase activity, thermal stability, impurity control and dosage form. Leveraging information gaps between domestic and international food regulations and process technologies, HiSiaddi collaborated with manufacturers to deliver end-to-end targeted customization, opening a direct supply channel for Chinese customized food enzymes into premium European dairy supply chains and achieving a win-win situation for overseas clients, domestic manufacturers and the foreign trade service provider.
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