HiSiaddi is an innovative foreign trade service provider driven by both technology transformation and foreign trade business. We have established a "1+2+3+4=1" service system and can supply ε-polylysine sourced directly from multiple well-known original manufacturers. As a foreign trade enterprise with independent R&D capabilities, HiSiaddi has repeatedly leveraged technical cooperation with manufacturers and accurate insight into market demands to resolve customized ε-polylysine requirements for clients. Below is a case study of HiSiaddi addressing customized ε-polylysine product demands.
Please contact HiSiaddi customer service if you require more customized service solutions.
The client is BioPur of Germany, a top European group specializing in organic food and clean label solution development. Its core product lines include premium organic cold fresh salad dressings, additive-free plant-based yogurts, Michelin-grade ready-to-eat meals, and puree complementary foods for infants. Products are sold to high-end supermarkets and organic certified specialty stores across Germany, Austria and Switzerland. The enterprise strictly complies with five major standards: EU EFSA, EC 1333/2008, EU Organic, Clean Label and Non-GMO. It imposes extremely stringent requirements on ε-polylysine (ε-PL): high purity with minimal impurities, precisely controlled degree of polymerization, low odor, compatibility with weak acid and emulsified systems, and direct applicability to infant food. This client belongs to the high-end segment with highly customized technical specifications and zero tolerance for product risks.
In February 2026, the client launched R&D for a new generation of clean label cold fresh salad dressing (pH 3.8–4.2, oil-in-water emulsified system), with core requirements as follows:
1. High purity & low sensitization: ε-PL purity ≥98%, free amino acids ≤0.5%, no fermentation odor, meeting infant food safety standards;
2. Precise polymerization degree control: molecular weight maintained at 3200–3800 Da (conventional products usually range from 4000–4500 Da) to balance bacteriostatic activity and emulsion stability;
3. System compatibility: bacteriostatic efficiency ≥95% under weak acid conditions at pH 3.8, no incompatibility with vinegar, egg yolk and vegetable oil in salad dressing, and no damage to emulsion structure;
4. Synergistic compounding: compound with 0.02% natamycin to jointly inhibit mold and Listeria, extending shelf life from 30 days to 90 days;
5. Compliant customization: provide English COA, batch traceability documents, organic certification and non-GMO statements in line with EU standards; support small-batch trial production (50kg per batch) and subsequent mass production (500kg per month).
The client previously contacted 3 domestic ε-PL manufacturers, all of which rejected the cooperation request due to insufficient technical capacity, long customization cycles, failure to meet target indicators, and lack of EU compliance experience:
· Manufacturer A: Only capable of producing conventional 4000–4500 Da products, unable to precisely regulate molecular weight;
· Manufacturer B: High-purity products carry strong odors, with bacteriostatic efficiency of only 70% under weak acid conditions; compounding with natamycin easily causes layering;
· Manufacturer C: No organic certifications available and unable to provide compliance documents for infant food.
The client urgently entrusted HiSiaddi with the project, requiring full completion of formula customization, lab-scale sampling, technical verification and compliance document processing within 15 days to launch exclusive tailor-made products from scratch.
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Difficulty in precise polymerization degree control Conventional ε-PL fermentation yields a wide molecular weight distribution (3000–4500 Da), while the client demands a narrow range of 3200–3800 Da. Standard fermentation processes cannot realize directional regulation; a molecular weight deviation exceeding ±200 Da will break the balance between bacteriostasis and emulsification performance.
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Challenges in high-purity, low-odor purification The client requires purity ≥98%, free amino acids ≤0.5% and complete elimination of fermentation odor. Conventional products only reach 90%–95% purity, containing residual bacterial protein and fermentation byproducts with heavy odors, making them unsuitable for infant food.
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Poor bacteriostatic stability under weak acid systems The salad dressing has a pH of 3.8–4.2; the bacteriostatic activity of standard ε-PL drops by 40%–50% when pH <4.0 due to interference from hydrogen ions, failing to hit the 95% bacteriostatic efficiency threshold.
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Weak compatibility with emulsified systems The formula contains vinegar, egg yolk and vegetable oil. Compounding conventional ε-PL with natamycin tends to destroy emulsion structures, resulting in layering, precipitation and astringent taste, damaging the quality of end products.
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Lack of infant-food-grade EU compliance documents A full set of English documents including EU Organic, Clean Label, Non-GMO, EFSA safety assessment and batch traceability reports is mandatory, yet most domestic manufacturers lack relevant certifications and complete document systems.
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HiSiaddi partnered with Jiangsu Yiming Bio, a domestic benchmark enterprise specializing in high-end customized ε-PL products with integrated fermentation and membrane separation technologies and complete EU certifications. A dedicated task force consisting of fermentation engineers, purification specialists, application technicians and compliance officers was assembled to complete technical decomposition within 24 hours:
1. Fermentation side: Screen high-yield Streptomyces albus strains, optimize fermentation parameters (temperature adjusted from 28℃ to 26℃, pH from 6.8 to 6.2, dissolved oxygen) to directionally synthesize polymers with molecular weight of 3200–3800 Da;
2. Purification side: Adopt a three-stage purification process combining enzymatic hydrolysis, nanofiltration membrane separation and vacuum low-temperature drying to remove bacterial protein, free amino acids and fermentation odors, lifting purity to ≥98%;
3. Application side: Adjust the surface charge of ε-PL molecules to enhance stability under pH 3.8–4.2 weak acid environments; optimize the compound ratio (0.05% ε-PL + 0.02% natamycin) to avoid emulsion breakdown;
4. Compliance side: Coordinate with SGS to conduct full-item testing per EU infant food standards and prepare a full set of English compliance documents concurrently.
Round 1 (3 days): Fermentation lab trials to lock polymerization degree Directional fermentation achieved molecular weight controlled at 3400±100 Da (within target range); Crude product purity hit 92% with free amino acids at 1.8% and slight residual odor, requiring purification optimization.
Round 2 (2 days): Purification optimization to boost purity and eliminate odors Enzymatic hydrolysis removes bacterial protein, nanofiltration membranes intercept impurities, followed by vacuum low-temperature drying at 60℃; Purity increased to 98.5%, free amino acids reduced to 0.3%, all fermentation odors eliminated, meeting infant food grade standards.
Round 3 (2 days): Compound application & system compatibility verification Simulate the client’s salad dressing formula (pH 4.0, vinegar + egg yolk + vegetable oil) with 0.05% ε-PL + 0.02% natamycin added; Bacteriostatic test: 98% inhibition rate against mold and Listeria (exceeding the 95% requirement); Stability test: No layering or precipitation after 90 days of static storage, with unchanged original flavor; Sensory test: Odor-free, no astringent taste, consistent with the taste requirements of high-end salad dressings.
5kg customized ε-PL samples were finally produced with core indicators as follows:
· Molecular weight: 3400 Da (within 3200–3800 Da target range)
· Purity: 98.5% (≥98% requirement)
· Free amino acids: 0.3% (≤0.5% requirement)
· Odor: Neutral, no fermentation odor
· Bacteriostatic efficiency under weak acid: 98% (≥95% requirement)
· Emulsion compatibility: Stable for 90 days without layering
Working with SGS compliance specialists, HiSiaddi prepared a full set of English documents complying with EU EFSA, EU Organic, Clean Label and Non-GMO standards:
1. Product qualification documents: EU food additive filing number for ε-PL, EFSA safety assessment report, EU Organic certification, Non-GMO statement;
2. Quality documents: English COA covering full-item testing (molecular weight, purity, free amino acids, heavy metals, microorganisms, solvent residues), batch traceability reports recording full processes from fermentation to packaging, HACCP/ISO 22000 certificates;
3. Application documents: Compound formula verification report, salad dressing stability test report, infant food safety assessment report.
1. Sample delivery: 5kg customized ε-PL samples attached with full English compliance documents, test reports, application schemes, compound ratios, addition procedures and precautions;
2. Technical handover: HiSiaddi technical team held video meetings with the client’s R&D department to explain fermentation processes, purification principles, molecular weight control logic, weak acid adaptation mechanisms, compounding skills, addition sequence and temperature control;
3. Client verification: The client conducted three rounds of small-batch production trials with the samples, all passing all tests:
o Bacteriostatic performance: No mold or Listeria growth within 90 days;
o Stability: No layering or precipitation over 90 days;
o Sensory performance: Pure flavor, delicate texture, no foreign odors;
o Compliance: Complete documents fully meeting EU infant food standards.
1. Small-batch trial production: The client placed an order of 50kg per batch; HiSiaddi coordinated priority production at the factory with delivery completed within 7 days;
2. Mass production planning: A long-term annual contract for 6 tons (500kg/month) was signed, with exclusive dedicated fermentation and purification production lines reserved at the factory to guarantee stable supply;
3. Technical support: HiSiaddi provides lifelong technical support, regularly tracking application performance, optimizing compound formulas and resolving technical issues arising during production;
4. Compliance guarantee: Real-time tracking of updates to EU regulations, annual certification reviews to ensure long-term product compliance.
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All customized indicators fully met and exceeded client expectations Molecular weight precisely controlled at 3400 Da within target range; purity reached 98.5% (≥98% threshold), free amino acids 0.3% (≤0.5% threshold); 98% bacteriostatic efficiency under weak acid conditions with stable emulsion for 90 days without layering; odor-free and compliant with infant food safety standards.
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Successful mass production of new products with outstanding market feedback The client’s new generation of clean label cold fresh salad dressing was officially launched and distributed to high-end German supermarkets. Sales volume rose 40% year-on-year within three months of launch. The product obtained EU Clean Label certification and became Europe’s first infant-grade additive-free salad dressing, greatly boosting the brand’s influence.
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High client recognition & binding long-term customized strategic cooperation Client feedback: "HiSiaddi is our most professional and trustworthy Chinese customized supply chain partner! Faced with five core challenges including precise molecular weight control of ε-PL, high-purity low-odor purification, weak acid stability, emulsion compatibility and EU compliance documents, they delivered end-to-end services from technical diagnosis and formula design to lab iteration, sample verification and compliance document processing within 15 days. The customized products perfectly fit our high-end application scenarios and enabled breakthrough development of our clean label new products, justifying deep long-term cooperation."
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The two parties subsequently signed an annual procurement agreement for 6 tons of high-end customized ε-PL. The client entrusted HiSiaddi with customized R&D, technical support, compliance audits and production capacity guarantee for all natural preservatives sourced from China, appointing HiSiaddi as its exclusive customized supply chain service provider in China. HiSiaddi will develop exclusive anti-corrosion solutions for subsequent new products including infant complementary foods and organic dairy products.
When overseas high-end clients purchase ε-polylysine for premium organic food, core customization pain points include difficulty in precise polymerization degree control, challenges in high-purity low-odor purification, poor bacteriostatic stability under weak acid environments, weak compatibility with emulsified systems and missing EU infant-food-grade compliance documents. High-end organic food imposes extremely stringent requirements on the safety, stability, compatibility and compliance of natural preservatives; standard off-the-shelf products cannot meet such demands and require full-link customized development covering fermentation, purification, application and compliance.
HiSiaddi’s core competitive advantages lie in "in-depth expertise in ε-PL technology, comprehensive mastery of EU high-end food standards, integration of top customized manufacturers, end-to-end technical services and long-term compliance guarantee": We master core fermentation and purification technologies for ε-PL to realize directional molecular weight regulation, purity improvement and odor elimination; we are well-versed in EU infant food regulations to prepare complete compliance documents; we also provide application technical support to resolve technical challenges including weak acid stability, emulsion compatibility and compound synergies. We deliver tailor-made products from scratch within 15 days, helping clients break technical bottlenecks, launch differentiated high-end products and seize market share in Europe’s clean label segment.
Please contact HiSiaddi customer service for more customized service solutions.
+86-13120989737 
info@hisiaddi.com 
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