Compound Non-Starch Polysaccharide Enzyme (NSP Enzyme)

Customization Case: Over 40% Enzyme Activity Inactivation at 85°C Pelleting & Enzyme Activity CV >15%

HiSiaddi is an innovative foreign trade service provider driven by dual engines of technology transformation and foreign trade services. It has established a "1+2+3+4=1" service system and can supply original factory products of multiple well-known brands for compound non-starch polysaccharide enzymes. As a foreign trade enterprise with R&D attributes, HiSiaddi has repeatedly resolved customized product demands for compound NSP enzymes through technological transformation cooperation with manufacturers and precise insight into market demands. Below is a case of HiSiaddi addressing customized requirements for compound non-starch polysaccharide enzymes.

Please contact HiSiaddi customer service if you need more customized service solutions.

1. Client Background

The client is De Heus from the Netherlands, a top-tier high-end European feed group specializing in high-end ruminant/monogastric premixes, organic feeds, and low anti-nutritional factor formulas. Its products cover core EU markets including the Netherlands, Belgium, Germany and Poland, targeting mid-to-high-end pastures and organic breeding bases. The company strictly complies with EU FAMI-QS, EFSA, GMP+, non-GMO, low-dust and traceability standards. Its core demands include precise adaptation to European wheat-rye based diets, high thermal stability, high batch consistency and in-depth customization. Its requirements for enzyme activity ratio, heat resistance, stress resistance and testing methods far exceed those of ordinary feed mills.

In January 2026, the client launched the "EU Low Anti-Nutritional Factor Feed Upgrade Project". Its core pain point: mainstream European wheat-rye diets contain high levels of arabinoxylan, β-glucan and pectin. Conventional commercial NSP enzymes feature crude formulations, insufficient heat resistance (over 40% activity loss after pelleting at 85°C) and large batch fluctuations (enzyme activity CV >15%), resulting in low feed conversion ratios, high faecal viscosity and numerous complaints from pastures. The client contacted three domestic enzyme manufacturers, all of which could only supply standard universal products and failed to conduct molecular-level customization tailored to raw material composition, pelleting processes and animal growth stages. Factories generally avoided actual measurement of thermal stability, batch consistency commitments and EU-standard testing. Trapped in the dilemma of "needing customized products yet unable to find qualified suppliers", the client urgently commissioned HiSiaddi, requiring full delivery of formula customization, lab trials, pilot trials and mass production within 45 days; otherwise, it would switch to high-priced European and American brands.

2. Three Major Customization Challenges (Core High-End Requirements)

2.1 Precise Formula Matching: Exclusive Enzyme Spectrum for Wheat-Rye Diets, Rejecting One-Size-Fits-All Solutions

Client diet composition: 60% wheat + 20% rye + 15% soybean meal + 5% miscellaneous meals, with primary anti-nutritional factors at 6.2% arabinoxylan, 1.8% β-glucan and 1.2% pectin.

Customization requirements: High-activity xylanase (endo- + exo-), moderate β-glucanase, appropriate pectinase and extremely low cellulase (to avoid degrading beneficial fibre); enzyme activity units accurate to 10 U/g with formulation error below 5%.

Manufacturer pain point: Factories only promote universal formulas combining xylanase, glucanase and cellulase, with no exclusive ratios developed for wheat-rye diets, and cannot make precise adjustments based on client raw materials.

2.2 High Thermal Stability: Withstand EU-Standard Pelleting at 85°C with Residual Enzyme Activity ≥70%

Client process: High-temperature pelleting (85°C for 3 min) followed by post-conditioning (75°C for 10 min), requiring residual enzyme activity ≥70% after pelleting (only 30–50% for conventional products).

Customization requirements: Enzyme molecular modification + carrier microencapsulation, fermentation with heat-resistant strains; residual xylanase activity ≥75% and β-glucanase ≥70% after 85°C/3 min treatment.

Manufacturer pain point: Lack of heat-resistant strains and microencapsulation technology; factories only mask activity loss by raising initial enzyme activity, leading to high costs and unstable efficacy.

2.3 Batch Consistency & EU Compliance: Enzyme Activity CV <8%, Full English FAMI-QS Documents & Traceability

Client standards: Inter-batch enzyme activity coefficient of variation CV <8% (over 15% for ordinary manufacturers); Eurofins EU-standard test reports, FAMI-QS certificates, non-GMO declarations, heavy metal/microorganism negative reports required for every batch.

Customization requirements: Standardized fermentation processes, enzyme activity testing methods aligned with EU EFSA standards, 25kg low-dust aluminium foil bags with English labels and traceable QR codes.

Manufacturer pain point: Extensive quality control loopholes, inconsistent national GB and EU testing methods, incomplete documents and non-compliant packaging, failing to meet the client’s zero-tolerance high-end standards.

3. HiSiaddi Customization Breakthrough: Closed-Loop Implementation Within 45 Days, Fully Aligned with Dutch High-End Demands

HiSiaddi assembled a team of enzyme formulators, thermal stability specialists, EU compliance officers and production quality control staff, cooperating with two domestic specialized, sophisticated, distinctive and innovative enzyme manufacturers (Xinhuyang and Andsu cooperative factories). We adopted a seven-step workflow: raw material analysis → formula design → thermal modification → lab trial verification → pilot scale-up → mass production quality control → compliant delivery, with full digitalization, traceability and transparency. The client could view progress and test data in real time.

Step 1: In-Depth Raw Material Analysis to Confirm Precise Anti-Nutritional Factor Data

Cooperating with Wageningen University in the Netherlands, HiSiaddi conducted molecular-level anti-nutritional factor testing on wheat, rye and soybean meal samples provided by the client:

· Arabinoxylan: 6.2% (2.8% soluble)

· β-glucan: 1.8% (1.1% soluble)

· Pectin: 1.2% (high-methoxy type)

· Cellulose: 2.1% (low degradation demand)

Based on the data, we prioritized core enzymes in the order: xylanase (primary) → β-glucanase (secondary) → pectinase (auxiliary), with restricted cellulase addition (≤5%) to prevent excessive fibre degradation.

Step 2: Molecular-Level Formula Design with Precise Activity Ratios & High-Specific-Activity Enzymes

Our formulators adopted a combination of high-specific-activity endo-xylanase + exo-xylanase + neutral β-glucanase + pectinase, with accurately calculated enzyme activity units:

· Xylanase (endo + exo): 12,000 U/g (high specific activity to degrade soluble and insoluble xylan)

· β-glucanase: 3,000 U/g (neutral pH compatible with intestinal tracts to break down β-glucan)

· Pectinase: 1,500 U/g (formulated for high-methoxy pectin)

· Cellulase: ≤500 U/g (restricted dosage to protect dietary fibre)

· Carrier: Corn starch + silicon dioxide (low dust, high fluidity)

Formula strengths: Fully matched to wheat-rye diets, precise enzyme activity, no redundant enzymes and optimal cost efficiency. The client’s technical team reviewed and confirmed the formula is scientifically sound, highly targeted and superior to European and American equivalent products.

Step 3: Thermal Modification & Microencapsulation Technology to Solve the 85°C High-Temperature Pelleting Challenge

Coordinating with cooperative manufacturers, HiSiaddi deployed three core heat-resistant technologies:

1. Fermentation with heat-resistant strains: Heat-mutated Bacillus licheniformis for xylanase production, boosting molecular thermal stability by 30%.

2. Double-layer microencapsulation: Inner starch layer + outer sodium alginate layer, maintaining ≥90% encapsulation integrity after 85°C/3 min treatment.

3. Low-temperature post-processing: Low-temperature concentration of fermentation broth (45°C) + spray drying (inlet air 160°C / outlet air 75°C) to avoid enzyme activity loss from high temperatures.

Lab trial test results: After 85°C/3 min pelleting, residual xylanase activity reached 78%, β-glucanase 73% and pectinase 71%, far exceeding the client’s 70% minimum requirement.

Step 4: Lab Trials → Pilot Trials → Mass Production, Strict Control of Batch Consistency (CV <8%)

· Lab trial (50kg): Produced per formula and sent to Eurofins Netherlands laboratory for testing; enzyme activity, heat resistance, heavy metal and microorganism indicators all passed.

· Pilot trial (500kg): Simulated client pelleting process with three parallel production batches; enzyme activity CV hit 6.2% (below 8%), meeting batch stability standards.

· Mass production (5 tons per batch): Standardized fermentation, precise blending, online enzyme activity detection and automatic packaging. Retained samples tested per batch, with all data uploaded to the client’s system for full traceability.

Step 5: EU Compliance Documents & Customized Packaging for Seamless Access to the Dutch Market

HiSiaddi supplied a full set of English compliance documents and customized premium low-dust packaging:

Document List

FAMI-QS certificate, ISO 22000, GMP+, non-GMO declaration, Eurofins test reports, batch traceability reports, EFSA-compatible enzyme activity testing method verification reports.

Packaging

25kg aluminium foil bags with PE inner liners (low dust, moisture-proof); English labels listing enzyme types, activity levels, ratios, heat resistance temperature, shelf life, batch numbers, traceable QR codes and EU responsible party information.

Delivery

Transported via constant-temperature containers (15–25°C) to prevent enzyme activity loss during transit.

4. Implementation Outcomes: Mass Production & Delivery of Customized Products with Remarkable Pasture Efficacy

4.1 Core Indicators of Customized Products (All Met or Exceeded Standards)

· Enzyme activity ratio: Xylanase 12,000 U/g + β-glucanase 3,000 U/g + pectinase 1,500 U/g with error <3%.

· Thermal stability: Residual enzyme activity ≥75% post 85°C/3 min pelleting (client requirement ≥70%).

· Batch consistency: Enzyme activity CV = 5.8% (<8%).

· Compliance: Full FAMI-QS, non-GMO and Eurofins certification approval.

4.2 Pasture Application Efficacy (Client Field Test Data)

Tests conducted on two large-scale Dutch pastures (100,000 broilers, 5,000 finishing pigs):

· Feed Conversion Ratio (FCR): Reduced by 4.2% for broilers and 3.8% for finishing pigs.

· Faecal viscosity: Cut by 35%, lowering litter waste and environmental pressure.

· Mortality rate: Reduced by 1.5%, with significantly improved intestinal health.

Client feedback: Product efficacy outperforms equivalent European and American brands while cutting costs by 18%.

4.3 Long-Term Cooperation: Annual Procurement of 120 Tons with Exclusive Customization Rights

The client formally designated HiSiaddi as its exclusive supplier for customized NSP enzymes, with an annual procurement volume of 120 tons and a 2-year exclusive customization agreement signed. Follow-up plans include developing specialized NSP enzymes for ruminants and low-allergen enzymes for organic feed.

Quote from the Client Technical Director: "HiSiaddi is the only high-end customized enzyme partner we have found in China! Most Chinese manufacturers only sell standard products with little understanding of European diet characteristics and high-temperature pelleting processes. HiSiaddi combines in-depth expertise in China’s enzyme technology with full mastery of high-end EU feed standards. They delivered a full-cycle solution within 45 days, covering raw material analysis, formula design, thermal resistance technology, compliance documents and customized packaging. The results far exceeded expectations, helping us break the monopoly of European and American brands while achieving cost reduction and efficiency gains. They are truly a strategic partner."

5. Case Summary

For foreign mid-to-high-end clients purchasing compound NSP enzymes, core pain points lie not in pricing, but four high-end barriers: customization capability, thermal stability, batch consistency and EU compliance.

· Low-end clients: Focus solely on price and initial enzyme activity, accepting universal standard products.

· Mid-to-high-end clients: Demand precise diet adaptation, high-temperature pelleting tolerance, stable batch quality, complete compliance documents and full traceability, and are willing to pay a reasonable premium for customization and superior quality.

HiSiaddi’s core competitive advantage lies in an integrated three-in-one capability: Technology + Supply Chain + Compliance.

1. Technology: Cooperate with specialized, sophisticated, distinctive and innovative domestic enzyme manufacturers to integrate heat-resistant strains, microencapsulation technology and precise formula design to meet molecular-level customization demands of high-end clients.

2. Supply Chain: Full quality control across lab trials, pilot trials and mass production, strict control of batch consistency (CV <8%) and stable large-scale delivery.

3. Compliance: Expertise in EU FAMI-QS, EFSA, non-GMO and other regulations, supply of full English compliance documents and customized packaging for seamless market access in the EU.

Please contact HiSiaddi customer service for more customized service solutions.